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| 2 | | | Recombinant Adeno-Associated Virus (rAAV) |
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| 3 | | | Vector Production Services * |
| Surface Area (sq.cm) | CHOP & UPenn Rate | External Academic Rate | Industry Rate | Endotoxin-free Plasmid Required |
| 4 | | | Inventory Order (aliquots of 50ul or 100ul) |
| NA | $300.00 | $330.00 | $390.00 | NA |
| 5 | | | Screening Scale (construct screening n >3) |
| 850 | $1,500 | $1,650.00 | $1,950.00 | 0.5mg |
| 6 | | 1,700 | $2,700 | $2,970.00 | $3,510.00 | 0.5mg |
| 7 | | 4,250 | $4,000.00 | $4,400.00 | $5,200.00 | 1.0 mg |
| 8 | | 8,500 | $6,000.00 | $6,600.00 | $7,800.00 | 2.0 mg |
| 9 | | 21,250 | $13,000.00 | $14,300.00 | $16,900.00 | 5.0 mg |
| 10 | | 34,000 | $18,400.00 | $20,240.00 | $23,920.00 | 10.0 mg |
| 11 | | TBD | TBD | TBD | TBD | TBD |
| 12 | | | Customized Vector Finish and Fill |
| TBD | TBD | TBD | TBD | TBD |
| 13 | | | * Cost includes production and Genomic titer by ddPCR (ITR) |
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| 15 | | | Recombinant Lentivirus (rLV) |
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| 16 | | | Vector Production Services ** |
| Surface Area (sq.cm) | CHOP & UPenn Rate | External Academic Rate | Industry Rate | Endotoxin-free Plasmid Required |
| 17 | | | Inventory Order (aliquots of 50ul or 100ul) |
| NA | $300.00 | $300.00 | $390 | NA |
| 18 | | | Pilot Scale (Centrifugation) |
| 450 | $1,000.00 | $800.00 | $1,300 | 0.1 mg |
| 19 | | | Regular Scale (Sucrose Gradient) |
| 2,544 | $3,300.00 | $3,300.00 | $4,290 | 0.3 mg |
| 20 | | | Process Scale (GLP-like process0 |
| 7,632 | $12,000.00 | $12,000.00 | $15,600 | 1.5 mg |
| 21 | | TBD | TBD | TBD | TBD | TBD |
| 22 | | | Customized Vector Finish and Fill |
| TBD | TBD | TBD | TBD | TBD |
| 23 | | | ** VSVG pseudotyped with 3rd generation packaging system |
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| 26 | | | Viral Vector Quality Control |
| CHOP & UPenn Rate | External Academic Rate | Industry Rate | | |
| 27 | | | AAV Purity Check by SDS-PAGE and Silver Stain |
| $1,200 | $1,320 | $1,560 | | |
| 28 | | | AAV Purity Check by SDS-PAGE and Coomassie Stain |
| $500 | $550 | $650 | | |
| 29 | | | AAV Genome Titer by ddPCR (transgene-specific) |
| $800 | $880 | $1,040 | | |
| 30 | | | AAV Genome Titer by ddPCR (ITR) |
| $600 | $660 | $780 | | |
| 31 | | | AAV Genome Integrity Check by Alkaline Agarose Gel Electrophoresis |
| $800 | $880 | $1,040 | | |
| 32 | | | LVV Particle Titer by P24 ELISA |
| $1,000 | $1,100 | $1,300 | | |
| 33 | | | LVV Particle Titer by RT-PCR |
| $800 | $880 | $1,040 | | |
| 34 | | | LVV Transduction Titer on HEK293 by Flow Cytometry |
| $700 | $770 | $910 | | |
| 35 | | | LVV Transduction Titer on HEK293 by ddPCR |
| $1,000 | $1,100 | $1,300 | | |
| 36 | | $600 | $660 | $780 | | |
| 37 | | $400 | $440 | $520 | | |
| 38 | | $600 | $660 | $780 | | |
| 39 | | | Cesium level by Osmolality |
| $600 | $660 | $780 | | |
| 40 | | | DNA Vector Cloning Amplification and QC |
| CHOP & UPenn Rate | External Academic Rate | Industry Rate | | |
| 41 | | | Cloning and Full Sequence Confirmation |
| $1,500 | $1,650 | $1,950 | | |
| 42 | | | Plasmid Amplification (Endotoxin-free Mega) |
| $400 | $440 | $520 | | |
| 43 | | | Plasmid Amplification (Endotoxin-free Giga) |
| $800 | $880 | $1,040 | | |
| 44 | | | Sequence and Structure analysis by RE digestion |
| $200 | $220 | $260 | | |
| 45 | | | Sequence Confirmation of Whole Construct |
| $800 | $880 | $1,040 | | |
| 46 | | | Special Request on Vector Finish and Fill |
| Customized | Customized | Customized | | |
| 47 | | | Shipping & Handling (via FedEx Express) |
| CHOP & UPenn Rate | External Academic Rate | Industry Rate | | |
| 48 | | TBD | TBD | TBD | | |
| 49 | | CHOP & UPenn Rate | External Academic Rate | Industry Rate | | |
| 50 | | $200 | $220 | $260 | | |
| 51 | | TBD | TBD | TBD | | |
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