RVC Price

	Service and Scale	Yield	At Cost ***	EndoFree Plasmid Plasmid
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1	rAAV Production (FY22)
2	Service and Scale	Yield *	At Cost ***	EndoFree Plasmid Plasmid
3	Inventory Order (aliquots of 50ul or 100ul)	1E11 - 1E12 GC	$275.00	NA
4	Pilot	1E12 - 5E12 GC	$2,200.00	0.3 mg
5	Small	6E12 - 1E13 GC	$3,800.00	1.0 mg
6	Regular **	1E13 - 2E13 GC	$5,500.00	2.0 mg
7	Large **	3E13 - 5E13 GC	$9,500.00	5.0 mg
8	Mega **	6E13 - 1E14 GC	$20,000.00	10.0 mg
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10	*The estimated yields are based on historic data. Due to the nature of individual, transgene constructs, the yield is varied, and therefore is not guaranteed.
11	**A minimum titer of 1E+13 vg/ml can be achieved based on packaging efficiency of the transgene, which can result in lower volume.
12	*** Hosptial surchage will be added for non CHOP/Penn clients, at 10% Academic Rate and 30% Industry Rate
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15	Service and Scale	Process	At Cost **	EndoFree Plasmid Plasmid
16	rLV Production (FY22)
17	Production Scale	Process	At Cost *	EndoFree Plasmid Required
18	Inventory order (Mini Aliquot)		$275.00	NA
19	Custom rLV Production - Pilot Scale	Centrifugation	$700.00	0.1 mg
20	Custom rLV Production - Regular Scale	Sucrose Gradient	$3,000.00	0.3 mg
21	Custom rLV Production - Process Scale	GLP like process	$7,500.00	1.5 mg
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24	* Hosptial surchage will be added for non CHOP/Penn clients, at 10% Academic Rate and 30% Industry Rate
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27	QC Supportive Service Core FY19
28	Quality Contol Assay	At Cost *
29	Particle titer and Purity Check by Silver Stain (AAV)	600
30	Genome titer by qPCR (AAV)	600
31	Genome titer by ddPCR (AAV)	600
32	P24 ELISA (LVV)	600
33	HEK293 Transduction titer by Flow Cytometry (LVV)	400
34	HEK293 Transduction titer by ddPCR (LVV)	600
35	Endotoxin test by Endosafe® nexgen-PTS™	300
36	Bioburden test	200
37	Mycoplasma test	300
38	Cesium level by Osmolality	400
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41	Supportive Service	At Cost *
42	Cloning and Full Sequence Confirmation	1,500.00
43	Plasmid Amplification (Endotoxin-free Mega)	450.00
44	Plasmid Amplification (Endotoxin-free Giga)	700.00
45	Sequence and Structure analysis by RE digestion	200
46	Sequence Confirmation of whole construct	800
47	Special Request on Vector Finish and Fill	Customized
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49	Shipping & Handling (via FedEx Express)	At Cost *
50	Shipping charge - US East coast	250
51	Shipping charge - US west coast	300
52	Shipping charge - international	Variable **
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54	Consultation Charges	TBD by projects
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56	* Hosptial surchage will be added for non CHOP/Penn clients, at 10% Academic Rate and 30% Industry Rate.
57	** Shipping charge is based on the cost from shipping core.
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63	Concentrated and purified Lentiviral vector (VSVG psedotyped) will be manufactured at Research Grade. The estimated yields are based on historic data and packaging efficiency.Due to the nature of individual trangene constructs, yields vary and therefore titer is not guaranteed.