CHOP Research Vector Core Service List

	-	--	---	-----	------	-------
	Collaboration powered by Smartsheet   |   Report Abuse
1	Updated in December 2023
2	Recombinant Adeno-Associated Virus (rAAV)
3	Vector Production Services *	Surface Area (sq.cm)	CHOP & UPenn Rate	External Academic Rate	Industry Rate	Endotoxin-free Plasmid Required
4	Inventory Order (aliquots of 50ul or 100ul)	NA	$300.00	$330.00	$390.00	NA
5	Screening Scale (w/o ddPCR titer)	850	$1,200	$1,320.00	$1,560.00	0.5mg
6	Screening Scale	850	$1,500	$1,650.00	$1,950.00	0.5mg
7	Pilot Scale	1,700	$2,700	$2,970.00	$3,510.00	0.5mg
8	Small Scale	4,250	$4,000.00	$4,400.00	$5,200.00	1.0 mg
9	Regular Scale	8,500	$6,000.00	$6,600.00	$7,800.00	2.0 mg
10	Large Scale	21,250	$13,000.00	$14,300.00	$16,900.00	5.0 mg
11	Mega Scale	34,000	$18,400.00	$20,240.00	$23,920.00	10.0 mg
12	Customized Scale**	TBD	TBD	TBD	TBD	TBD
13	Customized Vector Finish and Fill	TBD	TBD	TBD	TBD	TBD
14	* Cost includes production and Genomic titer by ddPCR (ITR)
15
16	Recombinant Lentivirus (rLV)
17	Vector Production Services **	Surface Area (sq.cm)	CHOP & UPenn Rate	External Academic Rate	Industry Rate	Endotoxin-free Plasmid Required
18	Inventory Order (aliquots of 50ul or 100ul)	NA	$300.00	$330.00	$390	NA
19	Pilot Scale (Centrifugation)	450	$1,000.00	$1,100.00	$1,300	0.1 mg
20	Regular Scale (Sucrose Gradient)	2,544	$3,300.00	$3,630.00	$4,290	0.3 mg
21	Process Scale (GLP-like process0	7,632	$12,000.00	$13,200.00	$15,600	1.5 mg
22	Customized Scale **	TBD	TBD	TBD	TBD	TBD
23	Customized Vector Finish and Fill	TBD	TBD	TBD	TBD	TBD
24	** VSVG pseudotyped with 3rd generation packaging system
25
26	Supportive Services
27	Viral Vector Quality Control		CHOP & UPenn Rate	External Academic Rate	Industry Rate
28	AAV Purity Check by SDS-PAGE and Silver Stain		$1,200	$1,320	$1,560
29	AAV Purity Check by SDS-PAGE and Coomassie Stain		$500	$550	$650
30	AAV Genome Titer by ddPCR (transgene-specific)		$800	$880	$1,040
31	AAV Genome Titer by ddPCR (ITR)		$600	$660	$780
32	AAV Particle Analysis by UV-VIS/DLS/SLS		$400	$440	$520
33	AAV Genome Integrity Check by Alkaline Agarose Gel		$800	$880	$1,040
34	LVV Particle Titer by P24 ELISA		$1,000	$1,100	$1,300
35	LVV Particle Titer by RT-PCR		$800	$880	$1,040
36	LVV Transduction Titer on HEK293 by Flow Cytometry		$700	$770	$910
37	LVV Transduction Titer on HEK293 by ddPCR		$800	$880	$1,040
38	Endotoxin test		$600	$660	$780
39	Bioburden test		$400	$440	$520
40	Mycoplasma test		$600	$660	$780
41	Cesium level by Osmolality		$600	$660	$780
42	DNA Vector Cloning Amplification and QC		CHOP & UPenn Rate	External Academic Rate	Industry Rate
43	Cloning and Full Sequence Confirmation		TBD	TBD	TBD
44	Plasmid Amplification (Endotoxin-free Mega)		$400	$440	$520
45	Plasmid Amplification (Endotoxin-free Giga)		$800	$880	$1,040
46	Sequence and Structure analysis by RE digestion		$200	$220	$260
47	Sequence Confirmation of Whole Construct		$800	$880	$1,040
48	Special Request on Vector Finish and Fill		TBD	TBD	TBD
49	Shipping & Handling (via FedEx Express)		CHOP & UPenn Rate	External Academic Rate	Industry Rate
50	Handling only (packaging and dry ice)		$50	$55	$65
51	US		$200	$220	$260
52	International		TBD	TBD	TBD
53	Consultation and CMC support		CHOP & UPenn Rate	External Academic Rate	Industry Rate
54	Hourly based		$200	$220	$260
55	Project-based		TBD	TBD	TBD
56
57
58
59
60
61
62